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Extracting viable reniform nematodes: an improved method

Permanent URL:
http://handle.nal.usda.gov/10113/11985
File:
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Abstract:
A reniform nematode isolation technique was established, which is based on an iso-osmotic density-gradient medium (Opti-Prep). This technique results in significantly higher numbers of clean eggs and vermiform nematodes that retain higher viability (48.6%) than samples processed with the sucrose method (28.7%). Nematodes survive exposure to OptiPrep for 22 hours without significant mortality whereas all nematodes died in the sucrose medium. OptiPrep provided a suitable, nontoxic alternative to the traditional density gradient material for the isolation of nematodes. This technique is convenient and relatively simple, with the added benefit of yielding cleaner samples compared to traditional isolation techniques. This is the first literature report of the use of the OptiPrep density-gradient medium for the isolation of nematodes. It could be expected to apply for extraction of other soil-borne nematodes.
Author(s):
Deng, D. , Zipf, A. , Tilahun, Y. , Sharma, G.C. , Jenkins, J. , Lawrence, K.
Note:
Conference held January 5-9, 2004, San Antonio, TX.
Source:
Proceedings of the Beltwide Cotton Conferences 2004
Language:
English
Year:
2004
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.