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A 610 kb YAC clone harbors 7cM of tomato (Lycopersicon esculentum) DNA that includes the male sterile 14 gene and a hotspot for recombination

Permanent URL:
http://handle.nal.usda.gov/10113/16004
File:
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Abstract:
Pollen development requires both sporophytic physic and gametophytic gene expression. We are using a map-based cloning technique to isolate sporophytic a genes which, when mutant, cause pollen abortion and a male sterile (ms) phenotype in tomato (Lycopersicon esculentum). We have genetically characterized one ms locus (ms14) using RFLP analysis and identified flanking markers. High-resolution genomic physical mapping indicates that the ms14 locus is located in a approximately 300 kb region. We have identified a YAC clone with an insert size of approximately 610 kb that contains the ms14-linked markers, reflects the organization of the physical map and therefore most probably contains the ms14 gene. In addition, we present evidence that the relationship between physical and genetic distance in this chromosomal region changes abruptly from approximately 105-140 kb/cM to less than 24 kb/cM, and suggest that the TG393-TG104 region is a hotspot for recombination.
Author(s):
Gorman, S.W. , Banasiak, D. , Fairley, C. , McCormick, S.
Note:
Includes references
Source:
MGG Apr 24, 1996. v. 251 (1)
Language:
English
Year:
1996
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.