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Exploiting synteny in Cucumis for mapping of Psm: a unique locus controlling paternal mitochondrial sorting

Permanent URL:
http://handle.nal.usda.gov/10113/20014
File:
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Abstract:
The three genomes of cucumber show different modes of transmission, nuclear DNA bi-parentally, plastid DNA maternally, and mitochondrial DNA paternally. The mosaic (MSC) phenotype of cucumber is associated with mitochondrial DNA rearrangements and is a valuable tool for studying mitochondrial transmission. A nuclear locus (Psm) has been identified in cucumber that controls sorting of paternally transmitted mitochondrial DNA. Comparative sequencing and mapping of cucumber and melon revealed extensive synteny on the recombinational and sequence levels near Psm and placed this locus on linkage group R of cucumber and G10 of melon. However, the cucumber genomic region near Psm was surprisingly monomorphic with an average of one SNP every 25 kb, requiring that a family from a more diverse cross is produced for fine mapping and eventual cloning of Psm. The cucumber ortholog of Arabidopsis mismatch repair (MSH1) was cloned and it segregated independently of Psm, revealing that this candidate gene is not Psm.
Author(s):
Al-Faifi, Sulieman , Meyer, Jenelle D. F. , Garcia-Mas, Jordi , Monforte, Antonio J. , Havey, Michael J.
Note:
Includes references
Source:
Theoretical and applied genetics 2008 Aug., v. 117, no. 4
Language:
English
Publisher:
Springer-Verlag
Year:
2008
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.