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Permanent URL:: http://handle.nal.usda.gov/10113/31361
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Abstract:: Water was added to solvent systems for thin layer chromatography of aflatoxins to achieve more reproducible results in lab. where temp. and RH vary. Resolution of the toxins also improved. Increments of water were added to solvent systems composed of acetone-chloroform (10 + 90, 12 + 88, and 15 + 85, v/v). As the water concn. was increased, separation of aflatoxins B2 and G1 improved. These 2 toxins are usually the most difficult to resolve in these solvent systems. Separations were the best with water-acetone-chloroform (1.5 + 12 + 88, v/v/v). Water added to methanol-chloroform (3 + 97, v/v) improved resolution of this solvent system but not enough for quantitative analysis, or at times, qualitative analysis. The solvent system water-methanol-ether (1 + 3 + 96, v/v/v) separated aflatoxins as well as water-acetone-chloroform (1.5 + 12 + 88, v/v/v).
Author(s):: Stubblefield, R.D. , Shannon, G.M. , Shotwell, O.L.
Note:: 20090714 20090801 00000000
Source:: Journal of the Association of Official Analytical Chemists July 1969, 52(4)
Language:: English
Publisher:: The Association
Year:: 1969
Collection:: Journal Articles, USDA Authors, Peer-Reviewed
Rights:: Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.