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Aflatoxin M1 in milk: evaluation of methods

Permanent URL:
http://handle.nal.usda.gov/10113/31550
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Abstract:
6 published methods for the determination of aflatoxin M1 in liquid and dried milk were compared because a quantitative assay sensitive to 0.1 mu g/l. was needed for routine analysis. Each method was tested with both spiked and naturally contaminated samples at levels of 0.1, 0.5, and 1 mu g/l. or 1, 5, and 10 mu g/kg; recoveries of M1 were determined. Data revealed that 2 methods, one for liquid milk and one for dried milk had the desired sensitivity and recoveries. In the liquid milk method M1 is extracted and milk protein is precipitated simultaneously with methanol-water (4+1); in the powdered milk method, M1 is extracted with acetone-water (70+30) and milk protein is then precipitated with a lead acetate solution. Both methods remove fats from the aqueous acetone or methanol solutions with hexane before partition of M1 into chloroform. Aflatoxin M1 is determined by TLC of the chloroform extracts and either visual or densitometric measurement of mycotoxin on thin layer plates. Modifications were made to simplify the methods and to reduce the time required to complete the assays.
Author(s):
Stubblefield, R.D. , Shannon, G.M. , Shotwell, O.L.
Note:
20090715 20090801 00000000
Source:
Journal of the Association of Offical Analytical Chemists Sept 1973, 56 (5)
Language:
English
Year:
1973
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.