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Expressed Sequence Tag Analysis of Eimeria-Stimulated Intestinal Intraepithelial Lymphocytes in Chickens

Permanent URL:
http://handle.nal.usda.gov/10113/37533
File:
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Abstract:
Intraepithelial lymphocytes (IELs) play a critical role in protective immune response to intestinal pathogens such as Eimeria, the etiologic agent of avian coccidiosis. A list of genes expressed by intestinal IELs of Eimeria-infected chickens was compiled using the expressed sequence tag (EST) strategy. The 14,409 ESTs consisted of 1851 clusters and 7595 singletons, which revealed 9446 unique genes in the data set. Comparison of the sequence data with chicken DNA sequences in GenBank identified 125 novel clones. This EST library will provide a valuable resource for profiling global gene expression in normal and pathogen-infected chickens and identifying additional unique immune-related genes.
Author(s):
Min, Wongi , Lillehoj, Hyun S. , Ashwell, Christopher M. , Van Tassell, Curtis P. , Dalloul, Rami A. , Matukumalli, Lakshmi K. , Han, Jae Y. , Lillehoj, Erik P.
Note:
Includes references
Source:
Molecular biotechnology 2005 June, v. 30, no. 2
Language:
English
Year:
2005
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.