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Enzyme-linked immunomagnetic chemiluminescent detection of Escherichia coli O157:H7

Permanent URL:
http://handle.nal.usda.gov/10113/41366
File:
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Abstract:
E. coli O157:H7 is a pathogenic microorganism that has been implicated in numerous cases of foodborne illnesses. A variety of rapid methods exist that show promise for the presumptive detection of this pathogen without the immediate need for incubating test samples for hours to days in microbial enrichment and culture media. In recent years, highly sensitive chemiluminescence has become a more affordable and portable detection method. Chemiluminescent detection has been coupled with the selectivity of antibodies, magnetic microparticle separation/isolation, and enzymatic signal amplification in order to develop a rapid method, termed enzyme-linked immunomagnetic chemiluminescence (ELIMCL). This work presents the application of ELIMCL to the detection of E. coli O157:H7 in pristine buffered saline with a detection limit of 7.6x10³ for live cells in approx. 75 min assay time. The blocking agent casein and the surfactant Tween 20 were used to lower background luminescence and thus maximize signal-to-noise ratios. After 5.5 h of enrichment culture, ELIMCL was demonstrated to detect E. coli O157:H7 inoculated in ground beef at 10 CFU/g in a total assay time of about 7 h.
Author(s):
Gehring, Andrew G. , Irwin, Peter L. , Reed, Sue A. , Tu, Shu-I , Andreotti, Peter E. , Akhavan-Tafti, Hashem , Handley, Richard S.
Note:
Includes references
Source:
Journal of immunological methods 2004 Oct., v. 293, no. 1-2
Language:
English
Year:
2004
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.