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Factors affecting Agrobacterium tumefaciens-mediated gusA expression and opine synthesis in Gladiolus

Permanent URL:
http://handle.nal.usda.gov/10113/42284
File:
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Abstract:
Five tumorigenic strains of Agrobacterium tumefaciens were used to inoculate corms, cormels, plants grown in vitro, and seed-derived seedlings of several cultivars of Gladiolus. Tumors formed on 12% of the plant tissues inoculated, and 1% of these tumors synthesized either octopine or nopaline. A. tumefaciens-mediated beta-glucuronidase (GUS) expression showed 0.5% and 3.5% GUS expression for plants grown in vitro and regenerable callus, respectively. GUS expression ranged from 40% to 61% when A. tumefaciens was incubated with leaves from seedlings grown in the dark, whereas leaves from seedlings grown under a 16-h light photoperiod showed no GUS, indicating the significant effect of etiolation on transient GUS expression mediated by A. tumefaciens.
Author(s):
Kamo, K.
Note:
Includes references
Source:
Plant cell reports Mar 1997. v. 16 (6)
Language:
English
Year:
1997
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.