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Evolution of chromosome 6 of Solanum species revealed by comparative fluorescence in situ hybridization mapping

Permanent URL:
http://handle.nal.usda.gov/10113/47639
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Abstract:
Comparative genetic linkage mapping using a common set of DNA markers in related species is an important methodology in plant genome research. Here, we demonstrate a comparative fluorescence in situ hybridization (FISH) mapping strategy in plants. A set of 13 bacterial artificial chromosome clones spanning the entire length of potato chromosome 6 was used for pachytene chromosome-based FISH mapping in seven distantly related Solanum species including potato, tomato, and eggplant. We discovered one paracentric inversion and one pericentric inversion within specific lineages of these species. The comparative FISH mapping data revealed the ancestral structure of this chromosome. We demonstrate that comparative FISH mapping is an efficient and powerful methodology to study chromosomal evolution among plant species diverged for up to 12 million years.
Author(s):
Lou, Qunfeng , Iovene, Marina , Spooner, David M. , Buell, C. Robin , Jiang, Jiming
Note:
Includes references
Source:
Chromosoma 2010 Aug., v. 119, no. 4
Language:
English
Publisher:
Springer-Verlag
Year:
2010
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.