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Development of monoclonal antibodies specific for Ricinus agglutinins

Permanent URL:
http://handle.nal.usda.gov/10113/55932
File:
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Abstract:
Ricin is a highly toxic, dichain ribosome-inactivating protein present in the seeds of Ricinus communis (castor), grown principally as a source of high quality industrial lubricant and as an ornamental. Because of its presence in industrial byproducts and its documented use for intentional poisoning, there is a need for analytical methodology to quantify ricin in both castor extracts and food matrices. We developed a panel of monoclonal antibodies to ricin, with most having strong cross-reactivity with RCA-1, a homologous but less toxic castor agglutinin. Some of the IgM-producing hybridomas appeared to produce a second IgG isotype and were further analyzed by fluorescence-activated cell sorting. The antibodies were effective in various ELISA formats, many with IC50’s in the range of 0.1-10 ng/mL and minimal matrix effects in skim milk. Assay specificity can be adjusted for analytical needs by varying the combination of antibodies in a sandwich ELISA format.
Author(s):
David L. Brandon , Bradley J. Hernlem
Note:
USDA Scientist Submission
Source:
Food and agricultural immunology 2009 v.20 no.1
Language:
English
Year:
2009
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.