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Detection of Ricin Contamination in Liquid Egg by Electrochemiluminescence Immunosorbent Assay

Permanent URL:
http://handle.nal.usda.gov/10113/55954
File:
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Abstract:
A monoclonal antibody-based electrochemical luminescence (ECL) method was developed for detecting and quantifying ricin in liquid egg, with a limit of detection of 0.2 ng/mL. Because this highly toxic protein, present in the seeds of Ricinus communis (castor), has been used for intentional poisoning in the past, it is important to have sensitive and reliable analytical methodology to detect ricin in food matrices such as liquid egg. The detection of this quantity of pure or crude ricin spiked into commercial samples of liquid egg provides approximately 50,000-fold greater sensitivity than required to detect a toxic dose of ricin (>1 mg) in a 100 g sample.
Author(s):
David L. Brandon , Anna M. Korn , Lily L. Yang
Note:
USDA Scientist Submission
Source:
Journal of food science 2011 v.77 no.4
Language:
English
Year:
2011
Collection:
Journal Articles, USDA Authors, Peer-Reviewed
Rights:
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.