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Prevalence of Toxoplasma gondii from free-range chickens (Gallus domesticus) from Addis Ababa, Ethiopia

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Prevalence of Toxoplasma gondii in free-range chickens (Gallus domesticus) is a good indicator of the environmental contamination with oocysts because chickens become infected mainly by feeding from ground/ feed/soil contaminated with oocysts. The seroprevalence of T. gondii antibodies in 125 free-range chickens from the Addis Ababa, Ethiopia was determined. Antibodies to T. gondii were assayed by the modified agglutination test; 48 (38.4 %) of 125 chickens were seropositive with titers of 1:5 in 14, 1:10 in 12, 1:20 in 14, 1:40 in 3, 1:80 in 1, 1:160 in 1, 1:320 in 1, and 1:640 or higher in 2 chickens. The hearts of 115 chickens were bioassayed for T. gondii infection. Hearts of 72 seronegative [MAT] < 1:5) chickens were pooled in 4 groups (20+18+19+15) and fed to 4 T. gondii-free cats; none of these 4 cats shed oocysts in their feces examined 3-21 days after feeding chicken tissues. Hearts of 43 seropositive chickens (MAT ≥ 1:5 or higher), were bioassayed individually in mice. Toxoplasma gondii was isolated from only 1 chicken with MAT titer of 1:80; the isolate was designated TgCKEt1; the isolate was not pathogenic for outbred mice. Restricted Fragment Length Polymorphism genotyping using 10 loci indicated the TgCKEt1 was ToxoDB PCR-RFLP genotype #1 (Type II clonal). Results of this study indicate environmental contamination with T. gondii oocysts around Addis Ababa.
G. Tilahun , N. Tiao , L. R. Ferreira , S. Choudhary , S. Oliveira , S. Verma K. , O. C.H. Kwok , B. Molla , W. J.A. Saville , G. Medhin , T. Kassa , H. Aleme , W. A. Gebreyes , C. Su , J. P. Dubey
USDA Scientist Submission
Journal of parasitology 2013 Aug. v.99 no.4
American Society of Parasitologists
Journal Articles, USDA Authors, Peer-Reviewed
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